Figure 2.

ACEK causes ER stress-induced disruption of arcuate axon outgrowth. Confocal images (A) and quantification (B) of the density of TUJ1 (neuron-specific class III beta-tubulin)-immunoreactive fibers extending from isolated arcuate nucleus (ARH) explants incubated with incubated with vehicle (saline), or aspartame (5 mM), or sucralose (5 mM), or ACEK (5 mM), or rebaudioside A, TUDCA alone (750 μg/ml), or TUDCA+ACEK (n = 6–8 explants per condition). Data are presented as mean ± SEM. *P < 0.05 vs. ACEK treated explants and ****P ≤ 0.0001 vs. control treated explants. Statistical significance was determined by one-way ANOVA followed by Bonferroni's Multiple Comparison test. Scale bars 100 μm.