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Ca2+-induced stomatal closure is significantly impaired in cpk33 mutants. Leaves of WT plants, cpk33-1, and cpk33-2 mutants with open stomata were exposed to the MES-KCl buffer containing different concentrations of CaCl2. Stomatal apertures were subsequently measured and presented as means ± SE of three independent experiments. Bars with different letters represent statistically significant differences.
