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CPK33 kinase activity is essential for strigolactone-induced stomatal closure. Leaves of WT, cpk33-1, cpk33-2, 35::CPK33 cpk33 #1, 35::CPK33 cpk33 #2, 35::CPK33K102R cpk33 #1, and 35::CPK33K102R cpk33 #2 plants with open stomata were exposed to the MES-KCl buffer in the absence or presence of 1 µM GR24. Stomatal apertures were measured and presented as means ± SE of three independent experiments. Bars with different letters represent statistically significant differences.
