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. 2019 Dec 17;10:835. doi: 10.3389/fendo.2019.00835

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Copyright © 2019 Long, Lu, Cheng, Zhan and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wnt signaling pathway was involved in NFPA. (A) Wnt signaling pathway was derived from PTMScan results of NFPAs compared to controls. The red color means upregulation of a molecule in NFPAs, and the green color means downregulation of a molecule in NFPAs. The gradient color degree means slightly different expression tendency of that molecule. (B) Activation of canonical Wnt pathway in NFPAs was verified by Western blot results of GSK-3α, GSK-3β, p-GSK-3β (Ser9), p-GSK-3α/β (Ser21/9), β-catenin and β-actin in control pituitaries (Con) and NFPAs (n ≥ 3). The relative intensity was the ratio of absorbance units of NFPAs compared to Con. *p < 0.05, **p < 0.01.