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. 2019 Dec 17;10:1272. doi: 10.3389/fneur.2019.01272

Figure 2.

Figure 2

VPS35 gene, protein structure and interactions. Retromer cargo recognition complex is formed by vacuolar sorting protein 26 (VPS26; 38 kDa), vacuolar sorting protein 29 (VPS29; 20 kDa), vacuolar sorting protein 35 (VPS35; 92 kDa); the dimer of sinexin (SNXs). The N-terminal region (in blue) from the amino acid residues 1 to 172 [1–172] and C-terminal region (violet) from 307 to 796 (307–796) are important, respectively, for the interaction with VPS26 and VPS29 (36). The amino acid residues involved in the interaction with the SNXs are at N-terminal region (1–35, 37–54) and C-terminal region (307–796). A structural level VPS35 is a right-handed α-helix solenoid, and it is predicted to have 34 helices, 13 of which are in C-terminal. Different missense mutations have been identified in fPD. VPS35 variant of uncertain significance (VUS) are localized between exon 9 (P316S), exon 13 (R542W), and exon 14 (I560T, H599R, M607V), and the confirmed pathogenic mutation (D620N) is localized on the exon 15 (genes in light violet).