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. 2019 Dec 23;9:19614. doi: 10.1038/s41598-019-56043-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dependence of AtSHMT2 and AtSHMT4 activity on pH. The initial velocity of the SHMT forward reaction was measured at different pH values with AtSHMT2 and AtSHMT4 (0.2 μM) using a fixed concentration of one substrate while varying the concentration of the other substrate. The fixed L-serine concentration was saturating. THF fixed concentration was giving the maximum activity when varying L-serine. All experimental points are the average ± standard deviation of three independent measurements. Panel (A) AtSHMT2, 30 mM L-serine; Panel (B) AtSHMT2, 180 µM THF; Panel (C) AtSHMT4, 30 mM L-serine; Panel (D) AtSHMT4, 30 µM THF at pH 6.5 and 7.0, 50 µM THF at pH 8.0, 80 µM THF at pH 8.5 and 100 µM THF at pH 9.5. Reactions were carried out at 30 °C.