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. 2019 Dec 23;9:19668. doi: 10.1038/s41598-019-56182-8

Table 1.

Primer sequences and product size for RT-qPCR analysis.

Gene Primer sequences Product size (bp) UPL Probe (Roche)
Amh F: 5′-GGG-GAG-ACT-GGA-GAA-CAG-C-3′ 67 41 (ref: 4688007001)
R: 5′-AGA-GCT-CGG-GCT-CCC-ATA-3′
Bax F: 5′-AGT-GTC-TCC-GGC-GAA-TTG-3′ 69 56 (ref: 4688538001)
R: 5′-CCA-CGT-CAG-CAA-TCA-TCC-T-3′
Bcl-XL F: 5′-TGA-CCA-CCT-AGA-GCC-TTG-GA-3′ 78 2 (ref: 4684982001)
R: 5′-GCT-GCA-TTG-TTC-CCG-TAG-A-3′
Becn1 F: 5′-GAC-TCG-ATT-TTG-TCT-TCC-GTA-CA-3′ 94 16 (ref: 4686896001)
R: 5′-CTG-GGT-TTT-GAT-GGA-ATA-GGA-G-3′
Bmp15 F: 5′-CAG-TAA-GGC-CTC-CCA-GAG-GT-3′ 113 21 (ref: 4686942001)
R: 5′-AAG-TTG-ATG-GCG-GTA-AAC-CA-3′
Gdf9 F: 5′-TTC-GTG-TGT-GCC-GGG-CAA-GT-3′ 196 SYBR Green
R: 5′-GTC-ACA-GGA-AGC-TCT-CTG-CCC-A-3′
Gapdh F: 5′-TGT-CCG-TCG-TGG-ATC-TGA-C-3′ 151 80 (ref: 4689038001)
R: 5′-GAG-TTG-CTG-TTG-AAG-TCG-CA-3′