Figure 1.

Transformation and in vitro regeneration of the soybean plants. (A): The cotyledons were infected by incubating with A. tumefaciens carrying the pBI121::GmDREB6 vector for 30 min; (B): Co-cultivation in the dark with CCM for 5 days; (C): The cotyledons were cultured in SIM multi shoot regeneration media supplemented with 2 mg/L BAP and 50 mg/L kanamycin for 2 weeks; (D): The cotyledons were removed and cultured on SEM supplemented with 0.5 mg/L GA₃, 0.1 mg/L IAA, and 50 mg/L kanamycin for 2 weeks; (E): Initiation of root growth in the regenerated shoots in RM supplemented with 0.1 mg/L IBA for 20 days; (F): The rooted plantlets were transferred to pots containing a mixture of rice husk char and sand in the ratio 1: 1.