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. 2019 Dec 23;9:19663. doi: 10.1038/s41598-019-55895-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression pattern of the GmP5CS gene in the four GM lines of the T2 generation and the non-transgenic plants (WT) as determined by qRT-PCR. Actin (152 bp) was used as the reference gene. WT: Wild-type; T2-2, T2-4, T2-7 and T2-10: GM soybean lines in T2 generation. The different letters above the columns indicate statistically significant differences (P < 0.05) as measured by Duncan’s tests. The data represents the mean of three biological replicates ± standard error.