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. 2019 Dec 23;9:19696. doi: 10.1038/s41598-019-56051-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WSB/EiJ mice show overall lower circulating inflammatory markers as compared to C57BL/6J mice, with no effect of diet. Compared analysis of main circulating cytokines and inflammatory markers in twelve week-old C57BL/6J and WSB/EiJ male mice either maintained under control feeding (CTRL), or challenged with HFD for the last 3 days of the period (3D) or during the whole 8-week experiment duration (8WK). (A) Interferon gamma (IFN-γ in pg/mL; N = 11–13 per group); (B) Keratinocyte-derived cytokine (KC/GRO in pg/mL; N = 10–14 per group); (C) Interleukin 5 (IL-5 in pg/mL; N = 11–13 per group); (D) Interleukin 10 (IL-10 in pg/mL; N = 10–14 per group); (E) Interleukin 6 (IL-6 in pg/mL; N = 11–12 per group); (F) Tumor Necrosis Factor alpha (TNF-α in pg/mL; N = 11–14 per group); (G) Interleukin 1 beta (IL-1β in pg/mL; N = 11–14 per group). (H) Total antioxidant status (TAS in mmol/L; N = 10–12 per group). Graphs represent medians using box and whiskers. Significant differences were indicated by different letters to account for between strain and between diet group differences (P ≤ 0.05; GLM).