Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 23;9:19696. doi: 10.1038/s41598-019-56051-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

WSB/EiJ mice show remarkably well-defined elongated ependymal cells in both the hypothalamic arcuate nucleus (ARC) and the hypothalamic paraventricular nucleus (PVN), whatever the diet is. Immuno-histochemical analysis of GFAP-positive cells (glial cells including astrocytes and tanycytes) in the hypothalamic nuclei of twelve week-old C57BL/6J and WSB/EiJ male mice either maintained under control feeding (CTRL), or challenged with HFD for the last 3 days of the period (3D) or during the whole 8-week experiment duration (8WK). (A) Representative confocal images of GFAP-positive cells (astrocyte- and tanycyte-like glial cells; in red) and DAPI-stained nuclei (in blue) in the ARC region of coronal sections of C57BL/6J (top panel) and WSB/EiJ (bottom panel) mice. GFAP-positive elongated tanycyte-like ependymal cells (pointed by white arrowheads) were present in the top region of ARC close to the third ventricle (3 v) at a much greater extent in WSB/EiJ sections than in C57BL/6J. In contrast, C57BL/6J sections showed a greater proportion of shorter and well distinguishable GFAP-positive astrocyte-like cells (pointed by yellow arrowheads; magnified in insets). (B) Representative confocal images of GFAP-positive cells (in red) and DAPI-stained nuclei (in blue) in the PVN region of coronal sections of C57BL/6J (top panel) and WSB/EiJ (bottom panel) mice. The effect of strain on the morphology of GFAP-positive cells (mostly astrocyte-like cells (yellow arrowheads) in C57BL/6J mice vs mostly elongated ependymal cells (white arrowheads) in WSB/EiJ sections) is even more pronounced in the PVN region than in the ARC. Scale bars in main images: 100 µm; scale bars in insets: 20 µm.