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The parasite out of fibroblast stained with Dil fluorescence microscopy in Ex/Em, 549/565 nm emission (Nikon Eclipse E200) (A). Free fibroblast stained by Dil with 12μm nucleus and 0.8 μm nucleolus stained red (B). The parasites are seen in the cytoplasm after 6 h of culture as red bodies that show the presence of labeled L. major within the fibroblast cytoplasm (C). They are able to enter the fibroblast nucleus 3 days after exposure and exit in 7th day (D).
