Skip to main content
. 2019 Nov 22;31:150–162. doi: 10.1016/j.molmet.2019.11.012

Figure 1.

Figure 1

Quantification of BrdU+cells in islets of partially pancreatectomized mice. (A) Overview of the experimental design. (B) A tissue section of the remnant pancreas excised 1 week after partial pancreatectomy stained with cresyl violet to reveal the islet core. The tissue was labeled with DAPI (green) and anti-BrdU antibodies (black) to detect the cell nuclei and replicating cells, respectively. (C) Enrichment of beta cell-specific markers shown by Q-PCR of mRNA extracted from laser capture microdissected islets. (D) Percentage of BrdU+ cells in the islet cores. The analysis included 7 mice, of which 4 were sham-operated and 3 were partially pancreatectomized. 40 slices/group were counted for BrdU+ cells. *p < 0.05.