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. 2019 Nov 22;31:150–162. doi: 10.1016/j.molmet.2019.11.012

Figure 3.

Figure 3

Effect of miR-132 on beta cell proliferation in EndoC-βH1 cells. (A–D) Representative confocal images of BrdU+ (red) and eGFP+ (green) in EndoC-βH1 cells transduced with adenovirus vectors for control eGFP (A and C) or miR-132-eGFP (B and D) expression either in 1.4 mM DMSO (A and B) or 10 μM harmine (C and D). Nuclei were detected with DAPI (blue). (E) BrdU+ and GFP+ EndoC-βH1 cells normalized to GFP+ cells in control or miR-132 overexpressing cells treated as described in (A-D). (F) Mean number of DAPI+ cells of EndoC-βH1 cells/field transduced as described in (A–D). White bars: cells transduced with CMV-eGFP adenovirus vector; black bars: cells transduced with CMV-miR-132/eGFP adenovirus vector. (G) Relative miR-132 level in EndoC-βH1 cells treated with 1.4 mM DMSO or 10 μM harmine. Data shown in (A–D) were collected from 15 to 20 images with 1,000–4,000 cells for each condition. Scale bars in (A–D) = 50 μm *p < 0.05, **p < 0.01, **p < 0.001.