Fig. 5.

Effects of ferroptosis inhibitors on ferroptosis of trophoblasts.

The in vitro PE model was established by culturing HTR-8/SVneo and TEV-1 cells under hypoxic conditions. Inhibitors of ferroptosis (60 nM Ferrostatin-1 and 100 μM Deferoxamine mesylate) were added to cells cultured under hypoxia. (A) Twenty-4 h later, the GSH content, GPx activity, labile Fe(II) concentration, and MDA content were measured in cells. (B) Intracellular ROS levels were measured using H2DCFDA probe through flow cytometer. (C)Western blot assay was performed to assess the levels of protein closely associated with ferroptosis. FIF: ferroptosis inhibitor, ferrostatin-1; FID: ferroptosis inhibitor deferoxamine mesylate. The mark “*” refers to significance between normoxia and hypoxia groups (*p < 0.05, **p < 0.01 and ***p < 0.001); The mark “#” indicates the significance in comparison of Hyp+FIF and Hyp+FID groups to hypoxia group (^#p < 0.05 and ^##p < 0.01). There is no significance between these groups if neither “*” nor “#” was observed above the bar.