Fig. 7.

The effects of miR-30b-5p, Pax3, and SLC7A11 on the viability, invasion, and ferroptosis of HTR-8/SVneo and TEV-1 cells.

The in vitro PE model was established by culturing HTR-8/SVneo and TEV-1 cells under hypoxic conditions. miR-30b-5p inhibitors, as well as Pax3 and SLC7A11 expression vectors were transfected into cells before the exposure to hypoxia. (A and B) RNA and protein levels of genes were assessed by PCR and western blot assays, respectively. (C and D) Cell viability and invasion capacity were evaluated by CCK-8 agent and transwell method, respectively. (E) GSH content, GPx activity, labile Fe(II) concentration, and MDA content were measured in cells. (F) Intracellular ROS levels were measured using H2DCFDA probe through flow cytometer. *p < 0.05, **p < 0.01, compared with the HTR-8/SVneo cells under normoxia; ^#p < 0.05, ^##p < 0.01, compared with the HTR-8/SVneo cells under hypoxia.