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. 2019 Dec 23;9:19636. doi: 10.1038/s41598-019-56209-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoblot analysis showing: (a) expression of Parg-GSTσ in P. argentipes kept at different temperatures, Lane A: + 4 °C, Lane B: + 37 °C, Lane C: + 55 °C; (b) Expression of Parg-GSTσ in H₂O₂ non-exposed Vs exposed P. argentipes, Lane A & B Non exposed, Lane C & D exposed; (c) Expression of Parg-GSTσ in 4% DDT exposed Vs non-exposed P. argentipes, Lane 1 & 4 show: DDT exposed (60 min & 30 min, respectively), Lane 2 & 3 show: Non exposed P. argentipes (60 min), Lane 5 & 6 show: Non exposed P. argentipes (30 min); (d) Carbonylation assay of DDT exposed resistant and susceptible P. argentipes shows more carbonylation in susceptible sandflies from S1-S3 in upper panel and lower panel shows loading control.