Fig 2.

Arthroscopic extraction of bone marrow shown step-by-step from the identification of the entry point of the trocar in the intercondylar notch to the aspiration of the bone marrow during the reconstruction of a right anterior cruciate ligament seen from the anterolateral portal with the patient on the supine position, except for (A), which is seen from the anteromedial portal. (A) Identification of the desired entry point at the femoral notch apex at 5 mm from the lateral edge of the PCL insertion. (B) At the desired entry point, insert a 14-gauge trocar through the anteromedial portal turning it clockwise. (C) Insert the trocar a depth of approximately 30 mm. (D) Remove the stylet, and attach a 10-mL syringe to discard the first milliliter of the aspirated bone marrow, then with a 30-mL syringe preloaded with 4 mL of a heparinized solution, proceed to aspirate slowly. (E) Turn the trocar 90° clockwise every 2 mL of aspirated bone marrow. (F) Withdraw the trocar 0.5 cm every 8 mL of aspirated bone marrow, until obtaining the 60-mL sample. In (A), the arrow is pointing to the desired entry point at the femoral notch apex. In (B) and (E), the arrows show the direction of the movement to insert the trocar. (PCL, posterior cruciate ligament.)