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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Oct 10;40(1):86–102. doi: 10.1161/ATVBAHA.119.313200

Figure 3. TLR4 and MyD88 regulate foam cell formation in response to agLDL but not acLDL.

Figure 3.

(A-D) Foam cell formation in response to agLDL was analyzed by confocal microscopy of (A) WT, (B) Tlr4−/− and (C) Myd88−/− BMMs treated with A546-agLDL for 12 hr, fixed and neutral lipids stained using LipidTOX green. (D) Confocal images were used to quantify LipidTOX intensity per field. (E-H) Foam cell formation in response to acLDL was analyzed by confocal microscopy of (E) WT, (F) Tlr4−/− and (G) Myd88−/− BMMs treated with acLDL (100 μg/mL) for 12 hr, fixed and neutral lipids stained using LipidTOX green. (H) Confocal images were used to quantify LipidTOX intensity per field. Data were compiled from 3 independent experiments per condition. Error bars represent the standard error of the mean (SEM). *** p ≤ 0.001. Scale bars 20 μm.

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