Figure 3. Treg/Th17 ratio and cells apoptosis was reversed by IL-6 Ab in insulin-resistant HepG2 cells.
(A,B,D) Ratio of Th17 and Treg cells was investigated. (C) Immunofluorescent staining of IL-17RC in IR group, IR+CAA group and IR+CAA+IL-6 AB group. (E) IL-17 concentration in IR group, IR+CAA group and IR+CAA+IL-6 AB group detected by ELISA. (F) Percentage of apoptotic HepG2 cells in IR group, IR+CAA group and IR+CAA+IL-6 AB group by flow cytometry assay. *P<0·05 versus IR group, #P<0·05 versus IR+CAA group.
