Figure 2. Circ-PVT1 knockdown enhanced PTX sensitivity in PTX-resistant gastric cancer cells.

(A) IC₅₀ value of PTX in MGC-803, MGC-803/PTX, AGS, and AGS/PTX cells. (B) Transfection efficiency of sh-circPVT1 in MGC-803/PTX and AGS/PTX cells. (C and D) Expression levels of P-gp and GST-π were detected by RT-qPCR in MGC-803/PTX and AGS/PTX cells. (E and F) P-gp and GST-π expression levels were assessed by Western blot in MGC-803/PTX and AGS/PTX cells transfected with Control, sh-NC and sh-circPVT1. (G) IC₅₀ value of PTX in transfected MGC-803/PTX and AGS/PTX cells. (H) Apoptosis rates were detected by flow cytometry assay in transfected MGC-803/PTX and AGS/PTX cells. (I) Invasion capability was assessed by transwell invasion assay in transfected MGC-803/PTX and AGS/PTX cells; *P < 0.05.