Figure 1.

(a) Western blot showing caveolin-1 protein levels (Cav-1, 22 kDa band) in WT sham and tMCAO ipsilateral (tMCAO Ipsi) and contralateral (tMCAO Contra) side to the lesion. α-Tubulin (50 kDa band) was used as loading control. Quantification was done using mean grey values and normalized against the mean value for Sham. n = 3 animals per condition. (b) Image showing the locations where pictures were taken for Cav-1 and cell-marker expression in relation to the lesion (dotted line showing loss of neuronal MAP-2 staining) on a coronal section in immunofluorescence analysis after tMCAO. (c–d) Cav-1 (green) co-localizes with CD31-labeled (red) endothelial cells in the striatum ipsilateral (c) and contralateral (d) to the ischemic lesion at 3 dpi. (e–f) In the ipsilateral (e) and in the contralateral hemisphere (f), Cav-1 (green) was also observed (arrowheads) in GS-positive astrocytes (red). (g–h) In the cortex, Cav-1 (green) was found to co-localize (arrows) in reactive astrocytes stained with GFAP (red). Respective control staining was done using the same markers on Cav-1 KO tissue and is available in Supplementary Figure 1. Scale bar = 20 µm.