Fig. 1.

VCR induces activation of CNS endothelial cells. Bend3 cells were incubated with Vincristine (100 nM) or saline control for 24 h. (A) Representative FACS profile showing the intensity of fluorescence for ICAM expression. (B) Quantification of ICAM expression (n = 3 independent experiments). (C) Representative FACS profile showing the intensity of fluorescence for VCAM expression. (D) Quantification of VCAM (n = 3 independent experiments). (E) Representative FACS profile showing the intensity of fluorescence for P-glycoprotein expression. (F) Quantification of PGP-1 expression (n = 3 independent experiments). (G) Trans-endothelial resistance (TEER) (n = 3 independent experiments). (H) Paracellular permeability expressed as permeability coefficient of bEND3 cells treated with VCR. Values are mean ± SEM. Statistical analysis was performed by using Student’s t test *p < 0.05 compared to saline treated cells.