Skip to main content
. 2019 Nov 25;20(23):5914. doi: 10.3390/ijms20235914

Figure 6.

Figure 6

Emetine inhibits the Wnt/β-catenin pathway in the cisplatin-resistant subpopulation of CL1-0 cells. (A) Cells in the cisplatin-resistant subpopulation of CL1-0 cells (CL1-0/CDDP) were treated with or without 120 nM emetine for 48 hours. The total protein expression of β-catenin, c-myc, and cyclin D1 and the nuclear expression of β-catenin were assessed by Western blotting. β-Actin and HDAC1 were used as internal controls. (B) The TOPflash (M50) reporter containing wild-type TCF/LEF binding sites produced a high level of transcriptional activity. The FOPflash (M51) reporter containing the mutated TCF/LEF binding sites was used as the negative control. The relative luciferase activity of TOPflash/FOPflash was analyzed after six hours of treatment with DMSO or the indicated concentration of emetine in the cisplatin-resistant subpopulation of CL1-0 cells (CL1-0/CDDP). The data are expressed as the means ± SDs from three independent experiments. ** p < 0.01, **** p < 0.0001 (Student’s t-test).