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Live/dead and Reactive Oxygen Species (ROS) staining of the bacteria attached to sample disks. After 6 h of culture, bacteria grown on TNS and TNS-heat continued to proliferate until they covered the surface; 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining was negative (Figure 6). However, bacteria grown on TNS-heat-UV were positive for DCFH-DA after 1 and 6 h. Scale bar = 200 µm.
