Table 1.
Disease evaluation design and the values of the wilt rate, DSI, and AUDPC for parents BVRC 25 and BVRC 1 and their progenies in response to root-dip inoculation with R. solanacearum strain Rs-SY1.
| Generations a | Experimental Design for Disease Evaluation Assays | Resistance Components | ||||
|---|---|---|---|---|---|---|
| Test × Block × Plants | No. of Plants | Inoculation Time | DSI b | Wilt Rate (%) c | AUDPC d | |
| Ps | 3 × 1 × 20 − 25 | 68 | Summer of 2016 | 3.98 ± 0.04 | 100 | 42.92 ± 1.84 |
| Autumn of 2016 | ||||||
| Summer of 2017 | ||||||
| Pr | 3 × 1 × 15 − 25 | 66 | Summer of 2016 | 0.31 ± 0.24 | 4.58 ± 3.95 | 1.68 ± 1.28 |
| Autumn of 2016 | ||||||
| Summer of 2017 | ||||||
| F1 (♀Ps × ♂Pr) | 3 × 1 × 15 − 25 | 68 | Summer of 2016 | 1.76 ± 0.17 | 48.59 ± 5.38 | 14.56 ± 3.08 |
| Autumn of 2016 | ||||||
| Summer of 2017 | ||||||
| BC1Ps | 1 × 1 × 31 | 31 | Summer of 2017 | 2.71 | 71.97 | 23.08 |
| BC1Pr | 1 × 1 × 33 | 33 | Summer of 2017 | 0.86 | 24.94 | 7.2 |
| F2 | 1 × 1 × 440 | 440 | Autumn of 2016 | 1.88 | 47.78 | 18.36 |
a Ps and Pr are the susceptible parent line BVRC 25 and resistant parent line BVRC 1, respectively. ♀ and ♂ represent female and male parent, respectively. b The disease severity index (DSI) was calculated at 20 days after root-dip inoculation based on a 0 to 4 rating scale as follows: DSI = ∑(disease score × number of plants with each disease score)/total number of plants. c Wilting was defined as DSI at 20 dpi of >2. d The area under the disease progress curve (AUDPC) was calculated from scores (0–4) evaluated at 7, 10, 15, and 20 dpi.