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. 2019 Nov 26;20(23):5948. doi: 10.3390/ijms20235948

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The expression of chicken PPARs were not activated by 17β-estradiol treatment in vivo and in vitro. The expression of three PPAR isoforms, including PPARα, PPARβ and PPARγ were quantified by qPCR. (a)The effect of 17β-estradiol on three PPAR isoforms mRNA expression in chicken liver after different concentrations of estrogen treatment for 12 h; (b,c) The expression of PPAR isoforms were not regulated by17β-estradiol in chicken primary hepatocytes and LMH cells after different concentrations of estrogen treated for 12 h. Each value is represented the mean ± SE (a, n = 10; b, c, n = 6). Student’s t test was used to determine the statistical significance.