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. 2019 Nov 22;20(23):5866. doi: 10.3390/ijms20235866

Figure 1.

Figure 1

Progranulin (Pgrn) protein content and secretion in murine TDP-43 loss-of-function (LOF) cells. (a) Representative Western blot (WB) images (left panel) of Pgrn protein in cell lysates (CELL) and conditioned media (CM) upon Tdp-43 knock-down (siTdp-43) in murine motoneuronal-like NSC-34 cells compared to negative control siRNA-transfected (siCTRL) cells. Densitometric analysis (right panel) shows Tdp-43 gene silencing efficiency. αTUBULIN was used for intracellular data normalization and as a non-secreted control protein in CM (mean ± s.e.m.; n = 5 independent experiments; two-tailed unpaired t test; *** p < 0.001). (b) Real time PCR of Grn gene expression upon Tdp-43 depletion (mean ± s.e.m.; n = 3 independent experiments; two-tailed unpaired t test). (c) Densitometric and statistical analysis of intracellular (CELL) Pgrn protein content shown in (a) (mean ± s.e.m.; n = 5 independent experiments; two-tailed unpaired t test; * p < 0.05). (d) Secreted Pgrn levels detected in CM by WB shown in (a) and by ELISA assays (mean ± s.e.m.; n = 4 independent experiments; two-tailed unpaired t test; ** p < 0.01).