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. 2019 Nov 22;20(23):5866. doi: 10.3390/ijms20235866

Figure 5.

Figure 5

Regulation of sortilin gene expression, splicing and protein levels in human and murine TDP-43 LOF cells. Real time quantification of total Sortilin and of the splicing isoforms (∆ex17b and ex17b) transcripts upon TDP-43 depletion by siRNA in (a) NSC-34 cells and (b) M17 cells (mean ± s.e.m.; n = 3 independent experiments; two-tailed unpaired t test; * p < 0.05, ** p < 0.01). Representative WB and densitometry analyses of intracellular sortilin protein levels in (c) NSC-34 and (d) M17 cells. Immunoblots with TDP-43 antibody were shown to assess gene silencing efficiencies. αTubulin was used for sample normalization (mean ± s.e.m.; n = 5 independent experiments; two-tailed unpaired t test; * p < 0.05). WB and densitometric data of sortilin protein detected in the conditioned media (CM) of e) NSC-34 and f) M17 cells knocked-down for TDP-43. αTubulin was used as a negative control in CM (mean ± s.e.m.; n = 4 independent experiments; two-tailed unpaired t test; * p < 0.05).