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. 2019 Nov 20;20(23):5826. doi: 10.3390/ijms20235826

Figure 1.

Figure 1

CALCRL expression is elevated at relapse of AML and in leukemic stem cells, and induced by araC. (a) Expression of CALCRL and RAMP1 in paired samples from diagnosis (Dx) and relapse (Rel) of AML was determined by qRT-PCR. Normalization to β-2-microglobulin and calibration to sample C1 was performed using the ΔΔCT method. C1–C4, healthy control samples 1–4; BM, bone marrow; MNC, mononuclear cells; P1–P11, patients 1–11. Means + SD from technical replicates. (b) Expression of CALCRL and RAMP1 in paired samples from diagnosis (Dx) and relapse (Rel), cultured in the presence or absence of araC for 2 days, was measured as in (a). (c) Expression of CALCRL in stem cell enriched (LSC+) versus stem cell depleted (LSC–) leukemic cell populations contained in data sets GSE76008 and GSE30377. (d) Expression of CALCRL in CD34+ cell populations from AML versus healthy donors, contained in data set GSE30029. (c,d) Data were used as provided in the respective data sets (log2 transformed or not). False discovery rate (FDR) was determined using the lmFit function of the R package Limma.