Figure 6.
CGRP increases drug resistance of CALCRL positive primary AML cells and is counteracted by olcegepant. (a) Expression of CALCRL in HNT-34 cells, healthy control BM MNCs, and primary human AML samples (P12–P15) was determined by qRT-PCR. Normalization to β-2-microglobulin and calibration to the control BM sample was performed using the ΔΔCT method. Means + SD from technical replicates. (b) CellTiter-Glo metabolic activity assay. Cells were pre-incubated first with 500 nM olcegepant or an equivalent amount of solvent for 15 min, next with or without 100 nM CGRP for 1 h, prior to addition of the indicated concentrations of araC or daunorubicin (DNR). Means + SD from technical replicates. Statistical tests were not performed because no biological replicates can be performed with patient samples, and statistical significance based on technical replicates is biologically meaningless.