Table 2.
Antibodies used for flow cytometry.
| Target | Clone | Fluorophor | Company | Dilution |
|---|---|---|---|---|
| Mouse Gr-1 | RB6-8C5 | AF700 | Biolegend | 1:100 |
| Mouse Gr-1 | RB6-8C5 | APC | Biolegend | 1:100 |
| Mouse CD11b | M1/70 | AF700 | Biolegend | 1:100 |
| Mouse CD3 | 17A2 | AF700 | Biolegend | 1:100 |
| Mouse B220 | RA3-6B22 | AF700 | Biolegend | 1:100 |
| Mouse Ter119 | TER119 | AF700 | Biolegend | 1:100 |
| Mouse c-Kit | 2B8 | APC-Cy7 | Biolegend | 1:50 |
| Mouse Sca-1 | D7 | PerCP/Cy5.5 | Biolegend | 1:50 |
| Mouse CD34 | MEC14.7 | PE/Cy5.5 | Biolegend | 1:50 |
| Mouse CD16/CD32 | 93 | PE/Cy7 | eBioscience | 1:50 |
| Mouse Ki-67 | 16A8 | APC | Biolegend | 1:50 |
AF700 labelled Gr-1, CD11b, CD3, B220, and Ter119 antibodies were combined to define Lin− cells.