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. 2019 Nov 23;20(23):5882. doi: 10.3390/ijms20235882

Figure 3.

Figure 3

The analysis of relative expression of genes encoding regulatory proteins involved in light signaling, such as CIB1 (a), CIB5 (b), PHR2 (c), PP7 (d), SUB1 (e), and COP1 (f), in WT dormant Arabidopsis (Col-0) seeds incubated for 20, 36, and 48 h on water in different light conditions (BL and white light of intensity = 180 µmol m−2 s−1, darkness) at temperature of 25 °C: The transcript level was normalized to reference genes (ACT7, APC2, and HBT) and to the internal control, which was the expression value of each particular gene observed in seeds incubated for 20 h in darkness. Three biological and two technical replicates were performed. The bars show the relative units ± SD, letters a-f indicate homogeneous groups for p ≤ 0.05.