Figure 7.
The relative expression of the XERICO gene in samples isolated from WT dormant Arabidopsis (Col-0) seeds incubated on water for 20, 36, and 48 h in different light conditions (BL and white light of intensity = 180 µmol m−2 s−1, darkness) at temperature of 25 °C (a): The transcript level was normalized to reference genes (ACT7, APC2, and HBT) and to the internal control, which was the expression value of XERICO gene obtained in darkness after 20 h of incubation. Three biological and two technical replicates were performed. The bars show the relative units ± SD, letters a-c indicate homogeneous groups for p ≤ 0.05. (b) The schematic presentation of the influence of light on GA and ABA metabolism and transduction of signal induced by these phytohormones during dormancy alleviation of imbibed seed: Light releases dormancy by modulation of expression of genes involved in GA and ABA metabolism, leading to changes in GA and ABA concentration and further in GA-dependent signal transduction via GID1, RGL, and XERICO genes.