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. 2019 Nov 25;20(23):5928. doi: 10.3390/ijms20235928

Figure 5.

Figure 5

RXR and PPARα enhance the WY-14643-induced increase in CYP1B1 expression in MCF-7 cells. (A) Effect of co-transfection of PPARα and RXR on the WY-14643-induced increase in CYP1B1 mRNA levels. MCF-7 cells transfected with PPARα and RXR were treated with WY-14643 (100–200 μM) for 24 h. Cells were lysed and total RNA was prepared for PCR analysis of CYP1B1 mRNA levels, relative to the level of GAPDH. (B) Effect of co-transfection of PPARα and RXR on WY-14643-induced CYP1B1 (−910/+25) promoter activity. MCF-7 cells were transfected with CYP1B1-Luc, PPARα, or RXR, and treated with WY-14643 (100–200 μM) for 24 h. Cells were harvested and assayed for luciferase activity. Bars are means ± standard deviations of three independent experiments performed in triplicate. * p < 0.01, significantly different from the control.