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. 2019 Nov 22;20(23):5859. doi: 10.3390/ijms20235859

Figure 1.

Figure 1

Downregulation of miR-29a, but not miR-29b or miR-29c in bupivacaine (BPVC)-injured group while compared with those in Sham control group (n = 6/each group). Sixty µL of 1.5% (w/v) BPVC were injected into gastrocnemius muscles of left hind (LH) limb to induce muscle ischemic injury, and gastrocnemius muscles collected from various designated time points were extracted the total microRNA and expression levels of miR-29a, miR-29b, or miR-29c were estimated by quantitative RT-PCR analysis. Of note, consistently suppression of miR-29a levels were found on (A) day 3 post BPVC-injury (initiated phase of angiogenesis [12]), (B) on day 5 post BPVC-injury (peak phase of angiogenesis progression [12]) or (C) on 3 weeks post injury (peak phase of fibrotic stage [1,45]) of muscle healing. Data were given as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 for the BPVC group vs. the Sham group.