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. 2019 Dec 2;20(23):6077. doi: 10.3390/ijms20236077

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of knocking down TASK-3 on cell cycle regulators and apoptosis. (A,B) The expression of cell cycle regulators in wild-type (WT) KATO III and MKN-45 cells, or the same cells transduced with an shRNA against GFP (shGFP) or TASK-3 (shBP9). (C) Cytometry analyses of apoptosis using annexin V and propidium iodide (PI). (D–G) Percentage of cells in early or late apoptosis based on percentages obtained in C. Error bars correspond to the mean ± SEM of three independent experiments. Cell cycle markers were evaluated by a two-way ANOVA test with a p ≤ 0.05. Early and late apoptosis were evaluated by ANOVA followed by Dunnett’s test. *** p <0.001, compared to WT. ns = no significant difference.