Figure 5.

ONC effect on the colony formation of the two A375 cell subpopulations. (a) Number of colonies produced in soft agar by A375P (blue bars) and A375DR (red bars) cells after 72 h incubation with or without 1 µM ONC. After ONC treatment, cells were detached, counted, and cultured for 21 days in the medium containing 0.6% agar. Data reported are relative to three independent replicates ± S.D. All differences detected were statistically significant, ** p < 0.01; and (b) Representative images of the colonies formed by A375P and A375DR cells in the presence or absence of ONC. 10× magnification images were taken with a Zeiss Axio Vert. A1 AxioCAM/cm⁻¹ 60N-C1”camera.