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. 2019 Nov 22;20(23):5871. doi: 10.3390/ijms20235871

Figure 2.

Figure 2

Relative TFF2 content in the high- and low-molecular-mass regions after non-reducing SDS-PAGE (Mc-577). (A,B) TFF2-containing fraction B12 after SEC of a human gastric extract (from Figure 1) was subjected to non-reducing 15% SDS-PAGE: Western blot analysis concerning TFF2 (A); Coomassie-stained part of the gel (B). Then, the high- (H) and low-molecular-mass regions (L1–L3) were cut out as indicated, proteins were eluted, and subjected to reducing SDS-PAGE. In addition, the remaining high-molecular-mass-samples not entering the gel were removed from the gel pockets (P). (C) Western blot analysis concerning TFF2 of P, H, and L1–L3 (reducing conditions).