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. 2019 Nov 26;20(23):5941. doi: 10.3390/ijms20235941

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Examinations of Rpl43 mutants. (A) The growth assays of RPL43-HA (PKL350) and Rpl43 mutants, rpl43ΔN-HA (PKL657), rpl43ΔC-HA (PKL658), rpl43ΔM-HA (PKL659), and rpl43-4C-HA (PKL660), in the GAL::RPL43 (KLY628) strain. Cells were normalized and spotted on Ura^-Gal and Ura^-Glc plates with serial dilution. (B,C) GST, GST-Rpl43 (PKL474), GST-Rpl43ΔN (PKL584), GST-Rpl43ΔC (PKL585), and GST-Rpl43ΔNH (PKL652) were used to interact with Puf6-His₆ (PKL56), ΔN100Puf6-His₆ (PKL512), and Loc1-His₆ (PKL586). (D) Immunoprecipitation of Brx1-TAP with the Rpl43 mutants. (E) The Rpl43 (color depicted as in Figure 3B) and Rpl2 (orange) are shown on the pre-60S (Gray). PDB: 4V7F.