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. 2019 Nov 26;20(23):5941. doi: 10.3390/ijms20235941

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Examinations of Loc1 mutants. (A) A diagram of the Loc1 truncation mutants. (B) LOC1-GFP (PKL337), loc1ΔC84-GFP (PKL449), loc1ΔC74-GFP (PKL450), loc1ΔC64-GFP (PKL451), loc1ΔN50-GFP (PKL655), and loc1ΔN100-GFP (PKL518) were transformed to loc1Δ (KLY218) and assayed via a growth test. (C) The localizations of Loc1 mutants were monitored using fluorescence microscopy. (D) GST, GST-Loc1 (PKL400), GST-Loc1ΔC84 (PKL580), GST-Loc1ΔC74 (PKL581), GST-Loc1ΔC64 (PKL653), GST-Loc1ΔN50 (PKL583), and GST-Loc1ΔN100 (PKL582) interacted with Puf6. (E) GST and GST-Rpl43 (PKL474) interacted with Loc1 (PKL586), Loc1ΔC84 (PKL588), Loc1ΔC74 (PKL589), Loc1ΔC64 (PKL592), Loc1ΔN50 (PKL650), and Loc1ΔN100 (PKL592). The positions of Loc1 mutants were indicated with *.