Table 2.
Plasmids used in this study.
| Plasmid | Gene | Relevant Marker | Source |
|---|---|---|---|
| PKL52 | PUF6-GFP | CEN LEU2 | This study |
| PKL53 | puf6ΔDE-GFP | CEN LEU2 | This study |
| PKL54 | puf6ΔPUF-GFP | CEN LEU2 | This study |
| PKL55 | puf6ΔNPUF-GFP | CEN LEU2 | [38] |
| PKL56 | pET28a-PUF6 | Kan | [38] |
| PKL85 | PUF6-myc | CEN LEU2 | [38] |
| PKL86 | puf6ΔD/E-myc | CEN LEU2 | This study |
| PKL87 | puf6ΔPUF-myc | CEN LEU2 | This study |
| PKL88 | puf6ΔNPUF -myc | CEN LEU2 | This study |
| PKL188 | puf6ΔN-GFP | CEN LEU2 | This study |
| PKL189 | puf6ΔN-myc | CEN LEU2 | This study |
| PKL198 | puf6(NLS)-myc | CEN LEU2 | This study |
| PKL199 | puf6(NLS)-GFP | CEN LEU2 | This study |
| PKL288 | pET28a-LOC1 | Kan | [38] |
| PKL306 | puf6ΔCPUF-myc | CEN LEU2 | This study |
| PKL333 | PUF6 | 2μ URA3 | This study |
| PKL337 | LOC1-GFP | CEN LEU2 | [38] |
| PKL349 | RPL43A-HA | CEN URA3 | This study |
| PKL350 | RPL43B-HA | CEN URA3 | [38] |
| PKL400 | GST-LOC1 | CEN LEU2 | This study |
| PKL449 | loc1ΔC84-GFP | CEN LEU2 | This study |
| PKL450 | loc1ΔC74-GFP | CEN LEU2 | This study |
| PKL451 | loc1ΔC64-GFP | CEN LEU2 | This study |
| PKL474 | GST-RPL43 | Amp | [38] |
| PKL512 | pET28a-puf6(PUF) | Kan | This study |
| PKL518 | loc1ΔN100-GFP | CEN LEU2 | This study |
| PKL563 | RPL43B-HA | CEN HIS3 | This study |
| PKL580 | GST-loc1ΔC84 | Amp | This study |
| PKL581 | GST-loc1ΔC74 | Amp | This study |
| PKL582 | GST-loc1ΔN100 | Amp | This study |
| PKL583 | GST-loc1ΔN50 | Amp | This study |
| PKL584 | GST-L43ΔC helix | Amp | This study |
| PKL585 | GST-L43ΔN coil | Amp | This study |
| PKL586 | pET28a-loc1ΔC84 | Kan | This study |
| PKL588 | pET28a-loc1ΔC74 | Kan | This study |
| PKL590 | pET28a-loc1ΔC64 | Kan | This study |
| PKL592 | pET28a-loc1ΔN100 | Kan | This study |
| PKL650 | pET28a-loc1ΔN50 | Kan | This study |
| PKL652 | GST-L43ΔN helix | Amp | This study |
| PKL653 | GST-loc1ΔC64 | Amp | This study |
| PKL655 | loc1ΔN50-GFP | CEN LEU2 | This study |
| PKL657 | rpl43BΔN-HA | CEN URA3 | This study |
| PKL658 | rpl43BΔC-HA | CEN URA3 | This study |
| PKL659 | rpl43BΔM-HA | CEN URA3 | This study |
| PKL660 | rpl43B-4C-HA | CEN URA3 | This study |
| PKL661 | MBP-KAP121 | Amp | This study |
| PKL662 | pET28a-puf6-Nter | Kan | This study |
| PKL663 | pET28a-puf6-Cter | Kan | This study |
| PKL746 | LOC1 | 2μ LEU2 | This study |