Figure 2.

Presence and characterization of the JC polyomavirus (JCV) NCCR DNA in NTC, polyps, and CRC pairs. (A) Migration on agarose gels of representative NCCR amplicons, obtained by nested PCR, as specified in the Methods section. M: marker; +: positive control; NTC: non tumor control; P: polyp; N: normal adjacent mucosa; T: tumor mucosa. (B) NCCR multiple alignment of sample sequences to JCV strains from the NCBI database. At the top, a schematic representation of the PML-type NCCR organization is reported. (C) Distribution of the Mad-1 prototype and Δ98 rearranged NCCR forms in NTC, polyps, and CRC, expressed as percentage of cases. See text for details.