Figure 2.

Morphology and immunofluorescence staining of astrocytes. (a) The cells had a star-shaped morphology with irregular protrusions after 3 days in the culture. (b) After 7 days, there were many protrusions on the surface of the astrocytes. (c) Pure astrocyte cultures were obtained after subculture. (a–c) Scale bar: 100 µm. (d) Immunocytochemical staining using green fluorescent dye showed that the astrocytes expressed glial fibrillary acidic protein (GFAP). All nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue fluorescent dye). Scale bar: 70 µm.