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Contribution of modifications to Con A aptamer affinity. (A) Binding curves of ConA-3-1 and ConA-3-1m to Con A and PSA from particle-based fluorescent measurements. (B) Binding activity for various ConA-3-1 derivatives in the presence of 10 nM Con A. Fluorescence intensities were normalized first to particle coating, then to the relative signal of ConA-3-1.
