Skip to main content
. 2019 Sep 18;14(12):2652–2662. doi: 10.1021/acschembio.9b00587

Figure 7.

Figure 7

ConA-3-1m is a potent inhibitor of Con A-induced hemagglutination. (A) We incubated various concentrations of ConA-3-1m with a human erythrocyte suspension containing 150 nM Con A, a concentration known to induce complete hemagglutination. The deposition of erythrocytes onto the bottom of the wells indicates inhibition of Con A activity. The positive control well contains only human erythrocytes, with no Con A. (B) Inhibition of hemagglutination, as measured by increased absorbance at 655 nm. We observed that ConA-3-1m inhibited 150 nM Con A with an IC50 of 95.0 nM. Error bars were derived from four replicates. (C–E) 40× microscopic images of normal human erythrocytes (C) and human erythrocytes incubated with (D) 0.65 μM Con A or (E) 0.65 μM Con A with 0.8 μM ConA-3-1m. Scale bars = 40 μm.