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. 2019 Nov 12;8(12):2710–2717. doi: 10.1021/acssynbio.9b00310

Figure 5.

Figure 5

Antimicrobials enhanced colon epithelial cell motility and wound healing. (A) Representative photomicrographs of the colon cell (Caco-2) migration assay after 48 h with phosphate buffer control (PBS), tegaserod (5-HTR4B agonist, 1 μM), serotonin (1 μM), and the five 5-HTR4B ligands (10 μM). For all photomicrographs for migration after 24 and 48 h, see Figure S4. (B) Quantification of the colon cell migration assay after 48 h. The cell migration elicited by revaprazan and hordenine is similar to that observed by serotonin and tegaserod, and it is statistically significantly different than the PBS control (P < 0.01). (C) Representative photomicrographs of the Caco-2 wound healing scratch assay after 48 h with control (PBS), tegaserod (1 μM), serotonin (10 μM), and the 5 identified 5-HTR4B ligands (10 μM). For all photomicrographs of the wound healing scratch assay after 24 and 48 h, see Figure S5. (D) Quantification of the colon cell wound healing scratch assay. The wound healing elicited by revaprazan, halofuginone, and hordenine is statistically significantly different than the PBS control (P < 0.05).