Skip to main content
. 2019 Nov 29;20(23):6025. doi: 10.3390/ijms20236025

Figure 8.

Figure 8

Effects of 67-kDa LR (LR) neutralization on ERK1/2 and pERK1/2 levels in the hippocampus of normal animals. The 67-kDa LR IgG infusion elevated pERK1/2 level in astrocytes, but not CA1 neurons and microglia, without altering ERK1/2 expression. U0126 attenuated pERK1/2, but not EKR, level in these cells following 67-kDa LR IgG infusion. (A) Representative photographs of negative control of ERK1/2 and p-ERK1/2 antibodies. (B) Representative photographs for pERK1/2 level in hippocampal astrocytes. (C) Quantitative values (mean ± S.E.M) of pERK1/2 level in hippocampal astrocytes (n = 7, respectively). Open circles indicate each value. Horizontal bars indicate the mean value. Significant differences are *,# p < 0.05 vs. control IgG and 67-kDa LR IgG, respectively (one-way ANOVA followed by Newman–Keuls posthoc test). (D) Representative photographs for pERK1/2 level in CA1 neurons (NeuN, a neuronal marker). (E) Quantitative values (mean ± S.E.M) of pERK1/2 level in CA1 neurons (n = 7, respectively). Open circles indicate each value. Horizontal bars indicate the mean value. Significant differences are *,# p < 0.05 vs. control IgG and 67-kDa LR IgG, respectively (one-way ANOVA followed by Newman–Keuls posthoc test). (F) Representative photographs for pERK1/2 level in hippocampal microglia (Iba-1, a microglia marker). (G) Representative photographs for ERK1/2 level in the hippocampal CA1 region.