Skip to main content
. 2019 Nov 29;20(23):6018. doi: 10.3390/ijms20236018

Figure 2.

Figure 2

HuArgI(Co)-PEG5000 inhibits motility in CRC cells. Caco-2 cell were treated with HuArgI(Co)-PEG5000 (100 pM) with or without L-citrulline (11.4 mM). Cell monolayers were wounded and images were taken at time 0 h and 72 h. (A) Representative wound closure images. The scale bar is 100 μm. (B) Frames were quantitated using ImageJ (National Institutes of Health, MA, USA). The width of each wound was measured at 11 different points. The average rate of wound closure was calculated and expressed as µm/h. (C) Caco-2 cells were untreated or treated with HuArgI(Co)-PEG5000 with or without L-citrulline for 72 h. Cells were then lysed and blotted for ASS-1 and Actin. (D) Quantitation of (C) using imageJ software, expressed as the fold change to control. Data are the mean ± SEM. p < 0.05 (* p < 0.05 and ** p < 0.005) indicates a statistically significant difference.