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. 2019 Nov 25;20(23):5924. doi: 10.3390/ijms20235924

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A2 — Examples of QuPath grading of cytoplasmic DAB intensity of three separate tumour cases. Each case has 4 separate panels presented (A–D). (A): High magnification (20×) image of immunohistochemistry staining of tumour. (B): QuPath markup of same region showing cell detection and classification. (C) Low magnification (4×) image of tumour, demarcated in red. (D): Low magnification image of tumour with QuPath markup. Markup: Green (stroma), Purple (immune cells), Blue (negative tumour cells).